Blood Typing Protocol

  1. Get 4 of the plastic trays and rinse them thoroughly. Label each tray with the name of the individual whose blood you are testing, using a wax pencil.
  2. Obtain a vial of simulated blood and place 2-3 drops of blood in each of the three wells (A, B, & Rh) of the correct plastic tray.
  3. Place 2-3 drops of each of the typing sera in the appropriate well of the tray (anti-A goes in the A well, anti-B in the B well and anti-Rh in the Rh well).
  4. Mix the blood and serum together with a toothpick. DO NOT USE THE SAME END OF THE TOOTHPICK IN TWO DIFFERENT WELLS!!! Always use a clean toothpick for mixing in a new well.
  5. Let the blood sit for approximately a minute and then begin examining the wells for agglutination reactions. If agglutination has occurred, you should see small dark spots within the well. These spots are easier to see against a white background, so put your tray over a blank sheet of paper or a paper towel.
  6. Record the blood type of this individual on the Blood Lab Worksheet.
  7. Repeat the above steps and determine the blood types for the simulated blood from each of the other three individuals. Again, record this information on the Blood Lab Worksheet.

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